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HIF HRE-Luc/LacZ Stable Expressing B16LS9 Mouse Melanoma Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Eye |
| Organism |
Mouse (M. musculus) |
| Donor History |
C57BL/6 mice ,Ocular melanoma |
| Growth Properties |
Adherent ,spindle shaped |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS + 1X Non-Essential Amino Acids (TM068) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 0.4 mg/ml Geneticin/G418 (G271) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/hif-hre-luc-lacz-stable-expressing-b16ls9-mouse-melanoma-cell-line.html |
HIF HRE-Luc/LacZ Stable Expressing B16LS9 Mouse Melanoma Cell Line expresses hypoxia-responsive dual LacZ/Luciferase reporter to monitor HIF-1 activity. This cell line is useful to study the effects of HIF-1 activity.
