Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
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Real-time PCR cyclery
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Pipetovací roboty
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Jednokanálové
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PCR, qPCR, RT
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Reverzní transkripce
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Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
GFP1-10 Stable Expressing MDCK Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Dog (Canine) |
| Donor History |
Female ,Adult |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Eagle's Minimal Essential Medium (EMEM) (TM511) + 5% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/gfp1-10-stable-expressing-mdck-cell-line.html |
GFP1-10 Stable Expressing MDCK Cell Line was established by transducing parental MDCK cells with the pCMV-GFP1-10 plasmid. By itself, GFP1-10 is a fragment containing three residues that constitute the GFP chromophore, thus is non-fluorescent. Therefore, it is valuable cell line to be used in assays in which GFP11 is fused to an epitope of a target protein to study. The fused protein may be transfected into the cell line. Upon complementation, GFP1-10 and GFP11 fluoresces and can be visualized by microscopy.
