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Bmal1 CRISPR Knockout EO771 Stable Cell Line – AT212
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Mammary |
| Organism |
Mouse (M. musculus) |
| Donor History |
Mammary gland of carcinoma patient ,C57Bl/6 |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 20 mM HEPES (TM058) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 0.3 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
The Mouse Bmal1 CRISPR Knockout EO771 Stable Cell Line is a cutting-edge tool for chronobiology and cancer research, particularly in the context of breast cancer. BMAL1, a core clock gene, plays a significant role in regulating circadian rhythms. This cell line facilitates exploration into how the absence of BMAL1 affects tumor growth, the tumor microenvironment, and response to treatment, shedding light on the intricate relationships between circadian biology and cancer. It offers a unique avenue to investigate potential therapeutic strategies targeting circadian mechanisms for cancer treatment, making it an invaluable resource for advancing our understanding of cancer biology and the development of chronotherapy.
