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Imunoanalýza
PINK1-YFP-IRES-DsRed Expressing HCT116 Stable Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Colon |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,58 ,Caucasian ,Colorectal carcinoma |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. McCoy's 5A Medium (TM506) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
The PINK1-YFP-IRES-DsRed Expressing HCT116 Stable Cell Line (T3510) was engineered to express the PTEN-induced putative kinase 1 (PINK1) tagged with Yellow Fluorescent Protein (YFP) and linked to DsRed through an Internal Ribosome Entry Site (IRES). PINK1 is a key mitochondrial serine/threonine kinase involved in mitochondrial quality control and autophagy. This cell line allows for the visualization of PINK1’s localization and behavior in response to mitochondrial damage, as it plays a crucial role in recruiting PARKIN to depolarized mitochondria to initiate mitophagy. This cell line is ideal for studying mitochondrial dynamics, autophagy, and the molecular mechanisms underlying Parkinson’s disease.Additional cell lines from this panel:Cat. T3511 – PINK1(F104M)-YFP-IRES-DsRed Expressing HCT116 Stable Cell Line
