DS19 Cells
| Unit |
5 x 10^6 cells/ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Mouse cell lines |
| Organism |
Mouse |
| Donor History |
Mouse erythroid leukemia |
| Growth Properties |
Suspension |
| Growth Conditions |
Culture medium: RPMI 1640 ,w: 2.0 mM stable Glutamine ,w: 2.0 g/L NaHCO3 (Cytion article number 820700a). Supplements: Supplement the medium with 10% FBS. Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
The DS19 cell line, often referred to as MEL DS19, represents an immortalized tumor cell line originating from murine erythroleukemia. This cell line was induced by the Friend virus complex (FVA virus), and it characteristically exhibits properties akin to those of proerythrocytes in their differentiation stage. DS19 cells are particularly noted for their utility in research focused on the molecular and cellular mechanisms underlying erythropoiesis and leukemogenesis.
One of the defining features of the DS19 cell line is its responsiveness to certain chemical agents such as dimethyl sulfoxide (DMSO) and hemin, which are known to induce differentiation in these cells. When treated with these agents, DS19 cells transition from a leukemic to a more normalized erythroid phenotype, mimicking stages of natural erythroid differentiation. This capacity for induced differentiation makes the DS19 cell line a valuable model for studying the regulation of erythroid differentiation, especially in contexts where this process is disrupted by leukemic transformation.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Třepačky
Suché lázně
Fluorometry
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Čtečky identifikačních čipů
Termočipy
Biochemie
Imunoanalýza

