HEL Cells

Unit

5 x 10^6 cells/ml

Cell Type

Tumor Cells

Tissue Type

Leukemia cell lines

Organism

Human

Donor History

Erythroleukemia

Growth Properties

Suspension

Growth Conditions

Culture medium: RPMI 1640

,

w: 2.0 mM stable Glutamine

,

w: 2.0 g/L NaHCO3 (Cytion article number 820700a). Supplements: Supplement the medium with 10% FBS. Dissociation reagent: Accutase. Freeze medium: As a cryopreservation medium

,

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

,

or CM-1 (Cytion catalog number 800100)

,

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

,

5% CO2

,

humidified atmosphere.

Immortalization Method
Full Information

https://www.cytion.com/HEL-Cells/305022

SKU: 305022 Kategorie: ,
Mgr. Otakar Fišer
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Mgr. Otakar Fišer
Produktový manažer - Life Science
Popis

HEL cells are a human erythroleukemia cell line that was established from the peripheral blood of a 30-year-old man with erythroleukemia in relapse after treatment for Hodgkin lymphoma in 1980.
HEL cells are capable of spontaneous and induced globin synthesis, producing mainly G gamma and A gamma chains. These cells also express embryonic chains (epsilon, zeta) and alpha chains in minimal amounts, while beta chains are undetectable.
HEL cells are round, large to occasionally giant polynucleated, single cells in suspension, with a few cells adherent. The expression of mutated JAK2 has been confirmed in these cells by RT-PCR and sequencing. HEL cells express several cell surface markers, including CD3-, CD13+, CD14-, CD19-, CD33+, CD41a+, CD71+, and CD235a+.
According to research, hydroxyurea, a medicine routinely used to treat a variety of cancers, including erythroleukemia, may also regulate the death of HEL cells.
HEL cell apoptosis produced by hydroxyurea may be connected to HEL cell terminal differentiation. Additionally, earlier research has shown that hydroxyurea may be crucial in controlling HEL cell proliferation and differentiation.

O značce

Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.

Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.