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Biochemie
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CHO-IL2RA Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Hamster cell lines ,Reproductive system cancer cell lines ,Transformed cell lines |
| Organism |
Chinese hamster |
| Donor History |
Chinese hamster ovary ,non-neoplastic; genetically engineered for IL2RA (CD25) surface expression |
| Growth Properties |
Adherent/suspension |
| Growth Conditions |
Culture medium: For adherent cultures: DMEM:Ham's F12 (1:1) ,w: 3.1 g/L Glucose ,w: 2.5 mM L-Glutamine ,w: 15 mM HEPES ,w: 0.5 mM Sodium pyruvate ,w: 1.2 g/L NaHCO3 (Cytion article number 820400a)For suspension cultures: CHO Growth Medium A (from InSCREENeX; InSCREENeX catalog number INS-ME-1039). Supplements: For adherent cultures: Supplement the medium with 5% FBS. Add Geneticin (G418-Sulfat) to achieve a final concentration of 0.5 mg/mL. Dissociation reagent: For adherent cultures: Trypsin-EDTA. Seeding density: 2 to 5 x 10⁴ cells/cm². Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. |
| Immortalization Method | |
| Full Information |
Disclaimer: The prices displayed for cell lines are exclusively for academic/not-for-profit customers. For commercial entitites the price is approximately €6,250. If you represent a commercial entity or are unsure which category applies, please
contact us.
CHO-IL2RA cells are recombinant Chinese hamster ovary (CHO) cells engineered to stably express human interleukin-2 receptor alpha (IL-2Rα; CD25/IL2RA), a high-affinity cytokine receptor subunit involved in regulation of T-cell activation and immune homeostasis. CD25 forms part of the heterotrimeric IL-2 receptor complex together with IL-2Rβ (CD122) and the common gamma chain (CD132), enabling high-affinity binding of interleukin-2 and activation of downstream JAK/STAT signaling pathways. Physiologically, CD25 is highly expressed on activated T lymphocytes and regulatory T cells (Tregs), and aberrant expression has also been reported in several hematologic malignancies and inflammatory disorders.
CHO-IL2RA cells are widely used in immunology and therapeutic development workflows for characterization of anti-CD25 monoclonal antibodies, cytokine-based therapeutics, bispecific antibodies, and engineered immune cell targeting strategies. The stable recombinant expression system enables quantitative assessment of ligand binding, receptor occupancy, antibody affinity, and receptor internalization. These cells are also valuable for flow cytometry assay development, potency testing, cell-based binding assays, and high-throughput screening applications involving IL-2 pathway modulation. In addition, CHO-IL2RA models can support studies examining selective targeting of activated T cells or regulatory T-cell-associated mechanisms in autoimmunity, transplantation, and cancer immunotherapy.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
