Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
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Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
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Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Čtečky identifikačních čipů
Termočipy
Biochemie
Imunoanalýza
KB Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Reproductive system cancer cell lines |
| Organism |
Human |
| Donor History |
Adenocarcinoma |
| Growth Properties |
Adherent |
| Growth Conditions |
Culture medium: EMEM (MEM Eagle) ,w: 2 mM L-Glutamine ,w: 2.2 g/L NaHCO3 ,w: EBSS (Cytion article number 820100a). Supplements: Supplement the medium with 10% FBS and 1% NEAA. Dissociation reagent: Accutase. Seeding density: 2 x 10⁴ cells/cm² will result in a confluent monolayer within 2 to 3 days. Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
The KB cell line is an adherent epithelial cell line initially believed to be derived from an epidermal carcinoma of the mouth. However, subsequent analyses, including isoenzyme assays, HeLa marker chromosome identification, and DNA fingerprinting, revealed that the KB cell line was actually established through contamination with HeLa cells. This misidentification underscores the importance of rigorous cell line authentication in research.
KB cells express keratin, a key structural protein in epithelial cells, as confirmed by immunoperoxidase staining. Additionally, they have been found to contain sequences from human papillomavirus 18 (HPV-18), which may be of interest in studies related to viral oncology. The isoenzyme profile of KB cells includes glucose-6-phosphate dehydrogenase (G6PD) type A, consistent with the characteristics of HeLa cells. Given these findings, it is critical to recognize that KB cells share many biological properties with HeLa cells, including the presence of HeLa-specific marker chromosomes.
As a result, KB cells should be used with caution, particularly in experiments where the exact cellular origin is crucial. Despite this, they remain a useful model for studying epithelial cell behavior, cancer biology, and the mechanisms of viral integration and expression. As with all cell lines, KB cells are intended strictly for in vitro research and are not suitable for therapeutic or in vivo applications.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
