RBE Cells

Unit

3 x 10^6 cells/ml

Cell Type

Tumor Cells

Tissue Type

Liver cancer cell lines

Organism

Human

Donor History

Intrahepatic cholangiocarcinoma

Growth Properties

Adherent

Growth Conditions

Culture medium: RPMI 1640

,

w: 2.0 mM stable Glutamine

,

w: 2.0 g/L NaHCO3 (Cytion article number 820700a). Supplements: Supplement the medium with 10% FBS. Dissociation reagent: Accutase. Freeze medium: As a cryopreservation medium

,

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

,

or CM-1 (Cytion catalog number 800100)

,

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

,

5% CO2

,

humidified atmosphere.

Immortalization Method
Full Information

https://www.cytion.com/RBE-Cells/305019

SKU: 305019 Kategorie: ,
Mgr. Otakar Fišer
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Mgr. Otakar Fišer
Produktový manažer - Life Science
Popis

The RBE cell line is a human intrahepatic cholangiocarcinoma (CC) cell line derived from a 64-year-old female patient. This cell line was established alongside a sarcomatoid counterpart (SSP-25) from the same tumor nodule, highlighting the coexistence of adenocarcinoma and sarcomatoid components within a single CC lesion. RBE cells are characterized by their epithelial morphology, growing as a monolayer with a Epithelial-like appearance, which is typical of epithelial cells.

Phenotypically, the RBE cell line expresses key markers associated with cholangiocarcinoma. These include cytokeratins CK7 and CK19, gamma-glutamyl transpeptidase (GGT), carcinoembryonic antigen (CEA), carbohydrate antigen 19-9 (CA19-9), and vimentin. Additionally, mucin production is detected in approximately half of the RBE cells, as evidenced by periodic acid-Schiff (PAS) staining. These features confirm the adenocarcinoma origin of RBE cells and distinguish them from the SSP-25 cell line, which lacks CEA, CA19-9, and mucin expression.

O značce

Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.

Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.