U-138 MG Cells

Culture medium: DMEM

w: 4.5 g/L Glucose

w: 4 mM L-Glutamine

w: 3.7 g/L NaHCO3

w: 1.0 mM Sodium pyruvate (Cytion article number 820300a). Supplements: Supplement the medium with 10% FBS. Dissociation reagent: Accutase. Seeding density: 1 x 10⁴ cells/cm². Freeze medium: As a cryopreservation medium

we use 50% basal medium + 40% FBS + 10% DMSO

or CM-1 (Cytion catalog number 800100)

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

5% CO2

humidified atmosphere.