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Acan CRISPR Knockout RCS Cell Line (Rat)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Bone |
| Organism |
Rat (R. norvegicus) |
| Donor History |
Swarm rat chondrosarcoma |
| Growth Properties |
Adherent and suspension ,polygonal and round |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) is recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 3.0 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/acan-crispr-knockout-rcs-cell-line-rat.html |
This parental Swarm rat chondrosarcoma (RCS) cell line features a stable knockout of aggrecan (Acan), mediated by CRISPR/Cas9 gene editing technology. This cell line is suitable for investigating the response of chondrocytes to the loss of aggrecan and its role in chondrosarcoma progression.
