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Imunoanalýza
Aifm1 CRISPR Knock Out Mouse L929 Cell Line – Clone T2-15-3
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Connective Tissue |
| Organism |
Mouse (M. musculus) |
| Donor History |
Male ,C3H/An ,100 days ,Derived from normal subcutaneous areolar and adipose tissue |
| Growth Properties |
Adherent ,fibroblast-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 7.0 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/aifm1-crispr-knock-out-mouse-l929-cell-line-clone-t2-15-3.html |
These adherent cells are derived from mouse fibroblasts and feature CRISPR mediated knockout of the Apoptosis Inducing Factor Mitochondria Associated 1 (AIFM1).
