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Ampullary Neuroendocrine Carcinoma Cell Line (NEC1452)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Colon |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,38 ,Rectal NEC |
| Growth Properties |
Suspension ,clumps |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow IV (TM004) + 10% FBS + 1% L-Glutamine (G275) + 10µg/ml insulin + 10mM nicotinamide + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method |
Tumor Cell Line |
| Full Information |
https://www.abmgood.com/ampullary-neuroendocrine-carcinoma-cell-line-nec1452-t8175.html |
NEC1452 originates from an ampullary neuroendocrine carcinoma, developed to enhance the understanding and treatment of aggressive neuroendocrine cancers. This cell line was meticulously created from patient tumor samples, followed by successful propagation in immunocompromised mice, leading to consistent PDX models. Unlike NEC913, NEC1452 does not express somatostatin receptor 2 (SSTR2) but shares similar high-grade characteristics with a Ki-67 index of 80-90%. The cell line maintains key neuroendocrine markers such as chromogranin A and synaptophysin, and exhibits critical genetic alterations in TP53 and RB1, mirroring the molecular profile of advanced neuroendocrine carcinomas. NEC1452 cells thrive in culture, both in suspension and as three-dimensional spheroids, providing a versatile platform for in vitro and in vivo studies. This model is particularly suited for exploring therapeutic avenues in cases where SSTR2-targeted treatments are not viable, thereby broadening the scope of neuroendocrine carcinoma research and therapeutic innovation.Additional cell lines from this panel: Cat. T8174 – Rectal Neuroendocrine Carcinoma Cell Line (NEC913)
