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Apolipoprotein d (Apod) Stable Knock-Out B16F10 Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Skin |
| Organism |
Mouse (M. musculus) |
| Donor History |
C57BL/6J mouse |
| Growth Properties |
Adherent ,Spindle-shaped |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% Heat-inactivated FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/Apolipoprotein-d-Apod-Stable-Knock-Out-B16F10-Cell-Line.html |
This B16F10 murine melanoma cell line features a stable knock-out of the Apolipoprotein d (Apod) gene. Genotype was confirmed by PCR and mRNAseq. Apod is involved in lipid transport and has been associated with Alzheimer’s Disease (AD), Parkinsons disease (PD) and stroke. Therefore, this cell line may serve as a useful for model for strudies investigating the pathology and treatment possibilities of such diseases.
