Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
ATG4A/B Stable Double Knockout HeLa Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Cervix |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,31 ,African American ,Cervial cancer |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 2 mM L-Glutamine (G275) + 1 mM Sodium Pyruvate (TM057) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
The ATG4A/B Stable Double Knockout HeLa Cell Line is engineered to lack both ATG4A and ATG4B genes, critical enzymes in the autophagic process. Through the use of CRISPR-Cas9, this cell line demonstrates a compounded impairment in autophagy, with drastically reduced processing of both LC3 and GABA type A receptor-associated protein-like 1 (GABARAPL1), although some residual autophagic activity remains due to the presence of ATG4C and ATG4D. The dual knockout nature of this cell line makes it a powerful model for studying the functional redundancy among ATG4 isoforms and the specific contributions of ATG4A and ATG4B to cellular homeostasis, autophagosome formation, and protein turnover.Complete list of cell lines from this panel:Cat. T3588 – ATG4B Stable Knockout HeLa Cell LineCat. T3589 – ATG4A/B Stable Double Knockout HeLa Cell LineCat. T3590 – ATG4A/B/C Stable Triple Knockout HeLa Cell Line
