Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Čtečky identifikačních čipů
Termočipy
Biochemie
Imunoanalýza
BHK-21 clone 13 Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Hamster cell lines |
| Organism |
Golden Hamster |
| Donor History | |
| Growth Properties |
Monolayer ,adherent |
| Growth Conditions |
Culture medium: EMEM (MEM Eagle) ,w: 2 mM L-Glutamine ,w: 2.2 g/L NaHCO3 ,w: EBSS (Cytion article number 820100a). Supplements: Supplement the medium with 10% FBS and 1% NEAA. Dissociation reagent: Accutase. Seeding density: 1 x 10⁴ cells/cm² will yield in a confluent layer in about 4 days. Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
BHK-21 clone 13 cells, a subline of the baby hamster kidney (BHK) cell line, have become a pivotal model in virology and molecular biology research due to their robustness, ease of culture, and high transfection efficiency. The cells are used in the study of virus infection, antigen production, and recombinant protein synthesis.
BHK-21 cells are susceptible to a broad range of viruses, including alphaviruses, flaviviruses, and rhabdoviruses, which has made them an invaluable tool in the study of viral replication, pathogenesis, and the development of viral vectors for gene therapy and vaccines. Their utility in viral research is further enhanced by their ability to support high-titer virus production, facilitating the study of virus-host interactions and the screening of antiviral compounds.
BHK-21 cells are further used in recombinant protein production because of their high transfection efficiency. This feature enables their utility for the production of therapeutic proteins, antibodies, and for the development of novel biotechnological products.
BHK-21 cells also serve as a model for studying cellular processes such as cell adhesion, signal transduction, and apoptosis. This has implications for understanding disease mechanisms and testing the cellular response to various stimuli, including drugs and environmental factors.
In summary, BHK-21 clone 13 cells serve as a critical tool in the fields of virology, molecular biology, and biotechnology.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
