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Imunoanalýza
BVRA Stable Knockout Hepa1c1c7 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Liver |
| Organism |
Mouse (M. musculus) |
| Donor History |
C57L mice ,Hepatoma |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. MEM α ,No Nucleosides Medium (TM512) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/bvra-stable-knockout-hepa1c1c7-cell-line.html |
BVRA (biliverdin reductase A) was knocked out of the mouse Hepa1c1c7 cells using sgRNAs inserted into the GuideIt-zsGreen plasmid. The plasmid was transfected and clones were selected via FACS sorting for fluorescence. Knockout was determined by qPCR and Western blot analysis. Studies have shown the loss of BVRA is associated with fat accumulation in livers of mice models. Therefore BVRA Stable Knockout Hepa1c1c7 Cell Line is useful to study the effects of BVRA and its association with obesity increased lipid availability and lipotoxicity in this liver cell line.
