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CAP1 Stable Knockdown AsPC-1 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Pancreas |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,62 ,Caucasian ,Ascites of a patient with cancer of the pancreas |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 250 µg/ml Geneticin/G418 (G271) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/cap1-stable-knockdown-aspc-1-cell-line.html |
CAP1 mediate extracellular signals to control cancer cell invasiveness. Using two shRNA constructs S2 and S3 that target independent nucleotide sequences and effectively silenced CAP1 in HeLa and breast cancer cells we were able to generate stable clones with efficient CAP1 knockdown in the PANC-1 and AsPC-1 cells as confirmed in Western blotting. Knockdown of CAP1 in cancer cells led to enhanced stress fibers as well as reduced cell motility and invasion into Matrigel. These cells can be used to investigated roles for CAP1 and its phosphor-regulation in pancreatic cancer cells. pRNA-U6.1/Neo vector was used S2 targets nucleotide 519-537 of human CAP1 gene; S3 targets nucleotides 1074-1092 of human CAP1 gene.
