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CHO-EGFR Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Hamster cell lines ,Reproductive system cancer cell lines ,Transformed cell lines |
| Organism |
Chinese hamster |
| Donor History |
Chinese hamster ovary ,non-neoplastic; genetically engineered for EGFR surface expression |
| Growth Properties |
Adherent/suspension |
| Growth Conditions |
Culture medium: For adherent cultures: DMEM:Ham's F12 (1:1) ,w: 3.1 g/L Glucose ,w: 2.5 mM L-Glutamine ,w: 15 mM HEPES ,w: 0.5 mM Sodium pyruvate ,w: 1.2 g/L NaHCO3 (Cytion article number 820400a)For suspension cultures: CHO Growth Medium A (from InSCREENeX; InSCREENeX catalog number INS-ME-1039). Supplements: For adherent cultures: Supplement the medium with 5% FBS. Add Geneticin (G418-Sulfat) to achieve a final concentration of 0.5 mg/mL. Dissociation reagent: For adherent cultures: Trypsin-EDTA. Seeding density: 2 to 5 x 10⁴ cells/cm². Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
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CHO-EGFR cells are recombinant Chinese hamster ovary (CHO) cells engineered to stably express human epidermal growth factor receptor (EGFR/ERBB1/HER1), a receptor tyrosine kinase belonging to the ErbB family. EGFR regulates key cellular processes including proliferation, survival, migration, and differentiation through activation of downstream signaling pathways such as MAPK/ERK, PI3K/AKT, and JAK/STAT. Aberrant EGFR expression, amplification, or mutation is frequently associated with multiple solid tumors, including non-small cell lung cancer, colorectal cancer, glioblastoma, and head and neck squamous cell carcinoma. Stable CHO-EGFR models provide a controlled platform for investigating receptor biology and therapeutic targeting.
CHO-EGFR cells are widely used in oncology research and biologics development for characterization of anti-EGFR monoclonal antibodies, tyrosine kinase inhibitors, bispecific antibodies, antibody-drug conjugates, and engineered immune cell therapies. These cells support quantitative assessment of ligand binding, receptor activation, internalization, phosphorylation status, downstream signaling, and therapeutic blockade. They are also commonly employed in flow cytometry assays, receptor occupancy studies, high-throughput screening, and potency testing workflows. Because CHO cells provide robust growth characteristics and relatively low endogenous expression of human receptor systems, they offer a reproducible background for recombinant EGFR expression and standardized assay development.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
