CLS-354 Cells

Culture medium: DMEM

w: 4.5 g/L Glucose

w: 4 mM L-Glutamine

w: 3.7 g/L NaHCO3

w: 1.0 mM Sodium pyruvate (Cytion article number 820300a). Supplements: Supplement the medium with 10% FBS. Dissociation reagent: Accutase. Seeding density: 1 x 10⁴ cells/cm² will yield in a confluent layer in about 6to7 days. Freeze medium: As a cryopreservation medium

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

or CM-1 (Cytion catalog number 800100)

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

5% CO2

humidified atmosphere.