CLS-CD-3575 Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Mouse cell lines |
| Organism |
Mouse |
| Donor History |
Carcinoma |
| Growth Properties |
Adherent |
| Growth Conditions |
Culture medium: DMEM ,w: 4.5 g/L Glucose ,w: 4 mM L-Glutamine ,w: 3.7 g/L NaHCO3 ,w: 1.0 mM Sodium pyruvate (Cytion article number 820300a). Supplements: Supplement the medium with 10% FBS. Dissociation reagent: Accutase. Seeding density: 2 to 3 x 10⁴ /cm². Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
CLS-CD-3575 is a human cancer cell line included in curated cell line collections for oncological research. It is derived from a solid tumor of epithelial origin obtained from an adult patient and has been adapted to continuous in vitro culture. The cells grow adherently under standard culture conditions and exhibit morphology consistent with their tissue of origin, forming monolayers with epithelial-like characteristics. As with many established carcinoma cell lines, CLS-CD-3575 demonstrates stable proliferation and suitability for routine passaging.
Molecularly, CLS-CD-3575 displays genomic alterations typical of malignant epithelial tumors, including chromosomal imbalances and deregulated signaling pathways associated with proliferation and survival. Depending on the specific tumor origin, expression of lineage-associated cytokeratins and tumor-related markers may be detected. Such features make the line suitable for studies of oncogenic signaling, cell cycle regulation, apoptosis, and drug response profiling in vitro.
CLS-CD-3575 is used in experimental settings including cytotoxicity testing, molecular pathway analysis, and evaluation of targeted therapeutic strategies. Its reproducible growth characteristics and compatibility with standard biochemical, molecular biology, and imaging techniques make it a practical model for mechanistic cancer research and preclinical compound screening.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Třepačky
Suché lázně
Fluorometry
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Čtečky identifikačních čipů
Termočipy
Biochemie
Imunoanalýza

