Colon-26 Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Mouse cell lines |
| Organism |
Mouse |
| Donor History |
Carcinoma |
| Growth Properties |
Adherent |
| Growth Conditions |
Culture medium: RPMI 1640 ,w: 2.0 mM stable Glutamine ,w: 2.0 g/L NaHCO3 (Cytion article number 820700a). Supplements: Supplement the medium with 10% FBS. Dissociation reagent: Accutase. Seeding density: 1 x 10⁴ cells/cm² will yield in a confluent layer in about 4 days. Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
The Colon-26 cell line, derived from a murine adenocarcinoma, was established following the induction of colon carcinoma in a BALB/c female mouse using N-Nitroso-N-methylurethane (NMU). This particular carcinogen was administered rectally, a method that efficiently models the initiation of colorectal cancer. The establishment of the Colon-26 cell line was first reported by Corbett et al. in 1975, marking a significant development in the study of carcinogen-induced cancers in animal models.
Colon-26 cells are transplantable and maintain the adenocarcinoma characteristics of the original tumor, making them a valuable tool for oncological research, especially in studies related to colorectal cancer. The cell line is particularly useful for examining the efficacy of anti-cancer therapies and the molecular pathways involved in colorectal cancer progression. Due to its origin in BALB/c mice, the Colon-26 cell line is also frequently used in immunologically relevant research, providing insights into the interaction between cancer growth and the immune response in a syngeneic host.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Třepačky
Suché lázně
Fluorometry
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Čtečky identifikačních čipů
Termočipy
Biochemie
Imunoanalýza

