Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
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Jednokanálové
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PCR, qPCR, RT
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Alergie
Intolerance
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Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
COS-7-DPA1*01:03/DPB1*02:01 Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Monkey (Primate) |
| Donor History |
Cercopithecus aethiops |
| Growth Properties |
Adherent ,fibroblast |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 1.0 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
The COS-7-DPA101:03/DPB102:01 cell line expresses HLA-DPA101:03 and DPB102:01 antigens, which are vital for antigen presentation to helper T-cells. This stable cell line was created using retroviral transduction and validated through flow cytometry. It is maintained without antibiotics, making it suitable for research into HLA class II molecule functions, immune response mechanisms, and the development of immunotherapies for autoimmune diseases.
