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Imunoanalýza
COS-7-DQA1*01:01/DQB1*05:01 Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Monkey (Primate) |
| Donor History |
Cercopithecus aethiops |
| Growth Properties |
Adherent ,fibroblast |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
The COS-7-DQA101:01/DQB105:01 cell line stably expresses the HLA-DQA101:01 and DQB105:01 antigens, which are crucial for presenting extracellular antigens to helper T-cells. Developed via retroviral transduction and validated using flow cytometry, this cell line is maintained without antibiotics. It is valuable for research on antigen presentation, immune system function, and the development of immunotherapies targeting HLA class II molecules in autoimmune diseases.
