Genetika člověka
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Biochemie
Imunoanalýza
COS-7-DQA1*01:02/DQB1*05:02 Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Monkey (Primate) |
| Donor History |
Cercopithecus aethiops |
| Growth Properties |
Adherent ,fibroblast |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
The COS-7-DQA101:02/DQB105:02 cell line expresses the HLA-DQA101:02 and DQB105:02 antigens, which are crucial for presenting antigens to helper T-cells. This cell line, developed via retroviral transduction, was validated using flow cytometry. It is maintained without antibiotics and is valuable for studying HLA class II molecule functions, immune response mechanisms, and the development of therapies for autoimmune diseases.
