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Biochemie
Imunoanalýza
COS-7-DQA1*05:01/DQB1*03:01 Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Monkey (Primate) |
| Donor History |
Cercopithecus aethiops |
| Growth Properties |
Adherent ,fibroblast |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 1.0 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
The COS-7-DQA*105:01/DQB1*03:01 cell line expresses HLA-DQA105:01 and DQB103:01 antigens, crucial for presenting antigens to helper T-cells. Engineered via retroviral transduction and validated using flow cytometry, this cell line is maintained without antibiotics. It is ideal for research on immune response dynamics, antigen presentation, and the development of therapies for autoimmune diseases and transplantation.
