Genetika člověka
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Biochemie
Imunoanalýza
COS-7-DRA1*01:01/DRB3*02:01 Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Monkey (Primate) |
| Donor History |
Cercopithecus aethiops |
| Growth Properties |
Adherent ,fibroblast |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
The COS-7-DRA101:01/DRB302:01 cell line stably expresses HLA-DRA101:01 and DRB302:01 antigens, crucial for antigen presentation and T-cell activation. Generated through retroviral transduction, this cell line was validated using flow cytometry. It is maintained without antibiotics and is valuable for immunological research, particularly in studying antigen presentation, T-cell interactions, and autoimmune diseases.
