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COX10 Stable Knockout Mouse Fibroblast Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Skin |
| Organism |
Mouse (M. musculus) |
| Donor History | |
| Growth Properties |
Adherent ,fibroblast |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1 mM Sodium Pyruvate (TM057) + 50 µg/ml Uridine + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/cox10-stable-knockout-mouse-fibroblast-cell-line.html |
To generate the COX10 Stable Knockout Mouse Fibroblast Cell Line a primary skin fibroblast culture was established from a knockin mouse homozygous for the floxed COX10 (exon 6). Cells were immortalized by retroviral infection of E6-E7 type 16 papiloma virus carrying G418 resistance. To generate COX10 knock-out; immortalized cells were transfected with plasmid expressing Cre recombinase and clones were obtained after hygromycin selection.
