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Imunoanalýza
CPS I and OTC Stable Expressing CHO (CHO-CPS45-22-OTC1-A19) Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Ovary |
| Organism |
Chinese Hamster (C. griseus) |
| Donor History |
Female ,Adult ,Chinese hamster |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. MEM α ,No Nucleosides Medium (TM512) + 10% FBS + 25 mM HEPES (TM058) + 1X HT Supplement + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 500 µg/ml Geneticin/G418 (G271) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/cps-i-and-otc-stable-expressing-cho-cho-cps45-22-otc1-a19-cell-line.html |
Carbamoyl phosphate synthetase I (CPS I) and ornithine transcarbamoylase (OTC), key enzymes in the urea cycle, were introduced to CHO cells by transfection with calcium phosphate to enable permanent CPS I and OTC gene expression. This cell line is suitable for use in the study of the urea cycle. Expression of these enzymes results in decreased ammonium ion production, making these cells ideal for research regarding the affect of ammonia levels on cell growth and productivity.
