CX-1 Cells

Unit

3 x 10^6 cells/ml

Cell Type

Tumor Cells

Tissue Type

Intestine cancer cell lines

Organism

Human

Donor History

Adenocarcinoma

Growth Properties

Adherent

Growth Conditions

Culture medium: DMEM

,

w: 4.5 g/L Glucose

,

w: 4 mM L-Glutamine

,

w: 3.7 g/L NaHCO3

,

w: 1.0 mM Sodium pyruvate (Cytion article number 820300a). Supplements: Supplement the medium with 10% FBS. Dissociation reagent: Accutase. Seeding density: 1 x 10⁴ cells/cm² will yield in a confluent layer in about 4to6 days. Freeze medium: As a cryopreservation medium

,

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

,

or CM-1 (Cytion catalog number 800100)

,

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

,

5% CO2

,

humidified atmosphere.

Immortalization Method
Full Information

https://www.cytion.com/CX-1-Cells/300159

SKU: 300159 Kategorie: ,
Mgr. Otakar Fišer
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Mgr. Otakar Fišer
Produktový manažer - Life Science
Popis

The CX-1 cell line is derived from a human colon adenocarcinoma, characterized by its metastatic potential, particularly to the liver when inoculated into suitable animal models such as athymic nude mice. CX-1 cells were generated by introducing HT-29 cells into athymic mice. These cells serve as a reliable model system for studying the intricacies of adenocarcinoma of the colon.
The CX-1 cell line expresses elevated levels of the carbohydrate antigens sialosyl Lewis a (sialosyl Le^a) and carcinoembryonic antigen (CEA), which are associated with tumor progression, metastasis, and adhesion to vascular endothelium in various cancers, including colorectal carcinoma.
The human colon carcinoma cell line CX-1 serves as a critical resource for understanding the molecular mechanisms of colorectal cancer metastasis.

O značce

Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.

Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.