EFO-27 Cells

The EFO-27 cell line is a human ovarian carcinoma model derived from a moderately differentiated serous papillary adenocarcinoma. It was established from a solid omental metastasis in a patient with advanced-stage ovarian cancer. EFO-27 is part of a series of ovarian tumor-derived cell lines developed to explore the hormonal regulation of ovarian cancer cell proliferation. In early passages, EFO-27 was reported to be aneuploid, with a modal chromosome number exceeding 100, indicating a high degree of chromosomal instability, a common feature of high-grade serous ovarian carcinomas.

EFO-27 cells display an epithelioid morphology in vitro and have been shown to form dome-like multicellular structures in monolayer culture, a phenotype sometimes associated with active ion transport and tight junction formation. In serum-free media, the proliferation of EFO-27 was stimulated by gonadotropic hormones, specifically human chorionic gonadotropin (hCG) and follicle-stimulating hormone (FSH), suggesting that the cells retain functional hormone receptor signaling pathways. This responsiveness highlights the potential role of gonadotropin signaling in promoting tumor growth and progression in ovarian carcinoma and supports EFO-27 as a relevant model for studying hormone-driven mechanisms in ovarian cancer biology.

EFO-27 has also been included in major multi-omics datasets, such as the Cancer Cell Line Encyclopedia (CCLE) and COSMIC, where its genomic profile contributes to drug sensitivity mapping and tumor subtype classification. These datasets provide additional layers of information, including gene expression, copy number alterations, and mutational landscape, positioning EFO-27 as a well-characterized resource for preclinical research in ovarian cancer.