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GFP Control AKR Cell Line (Lenti-CMV-CBH-GFP-2A-Puro-Blank )
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Esophagus |
| Organism |
Mouse (M. musculus) |
| Donor History | |
| Growth Properties |
Adherent |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 0.4 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/GFP-Control-AKR-Cell-Line-Lenti-CMV-CBH-GFP-2A-Puro-Blank-T9828.html |
The GFP Control AKR Cell Line, designated as Lenti-CMV-CBH-GFP-2A-Puro-Blank, is applicable for research across various scientific disciplines, particularly in genetics and cell biology. This cell line is derived from AKR mice, known for their susceptibility to leukemia, making it especially relevant for cancer research. Engineered to stably express Green Fluorescent Protein (GFP) through a lentiviral vector under the control of the CMV (Cytomegalovirus) promoter, it allows for straightforward visualization and tracking of cells in vivo and in vitro. The inclusion of the 2A-Puro-Blank selection marker facilitates the selection of transduced cells using puromycin, enhancing the efficiency of experimental workflows. This versatile cell line is indispensable for studies involving gene expression, oncology, cell migration, and interaction, providing critical insights into disease mechanisms and potential therapeutic targets.
