Genetika člověka
Mikrobiologické aplikace
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Biochemie
Imunoanalýza
GFP Control Huh-7 Cell Line (Lenti-CMV-CBH-GFP-2A-Puro-Blank )
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Liver |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,57 ,Japanese ,Hepatocellular carcinoma |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 1.4 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/GFP-Control-Huh-7-Cell-Line-Lenti-CMV-CBH-GFP-2A-Puro-Blank-T9827.html |
This GFP stably expressing Huh-7 cell line is an essential research tool for studying gene expression, protein localization, and cellular processes in hepatocellular carcinoma cells. The stable expression of GFP enables researchers to track and analyze cellular dynamics in real-time, making it ideal for live-cell imaging, monitoring gene expression, and validating transfection efficiency. This cell line is also beneficial for comparative studies where a fluorescent marker is needed as a control.
