Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
H295RA Cells
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,48 ,African American |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow IV (TM004) + 10% cosmic calf serum + 1% ITS Plus + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. Selection with 1 μg/mL blasticidin |
| Immortalization Method | |
| Full Information |
H295RA is an ACTH-responsive human adrenocortical cell line, produced from H295R cells that have been transduced to stable express increased human MRAP (MC2R accessory protein) gene. H295R adrenocortical cell line displays relatively low ACTH responsiveness due to low melanocortin 2 receptor (MC2R) expression. Successful induction of MRAP and increased expression of MC2R in H295RA cells was confirmed by quantitative real-time RT-PCR and protein analysis. This cell line is well-suited for research regarding molecular analysis of physiologic and pathologic conditions involving the hypothalamic–pituitary–adrenal axis.
