HEK293-CXCR4 Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Transformed cell lines |
| Organism |
Human |
| Donor History |
Transformed/immortalized; non-tumorigenic (HEK293 background) |
| Growth Properties |
Monolayer ,adherent |
| Growth Conditions |
Culture medium: RPMI 1640 ,w: 2.0 mM stable Glutamine ,w: 2.0 g/L NaHCO3 (Cytion article number 820700a). Supplements: Supplement the medium with 10% FBS ,1 mM sodium pyruvate ,10 mM HEPES ,1% NEAA. Add Geneticin (G418-Sulfat) to achieve a final concentration of 1 mg/mL. Dissociation reagent: Trypsin-EDTA. Seeding density: 2 to 4 x 10⁴ cells/cm². Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
Disclaimer: The prices displayed for cell lines are exclusively for academic/not-for-profit customers. For commercial entitites the price is approximately €6,250. If you represent a commercial entity or are unsure which category applies, please
contact us.
The HEK293-CXCR4 cell line is a stable recombinant HEK293 cell line engineered to express the CXCR4 receptor at a medium-high level, approximately 7,800 molecules per cell. This cell line was developed using inscreenex’s landing pad technology, which ensures precise and reproducible integration of the CXCR4 gene at a specific, pre-validated genomic locus. CXCR4, also known as CD184, is a chemokine receptor belonging to the G protein-coupled receptor (GPCR) family. It plays a crucial role in immune cell trafficking, hematopoiesis, and serves as a co-receptor for HIV entry into cells. CXCR4 is also implicated in tumor growth, metastasis, and angiogenesis, particularly in hematological malignancies, making it a significant target in cancer research and therapy development.
The expression of CXCR4 in this cell line was confirmed using flow cytometry with a target-specific antibody, ensuring consistent and reliable receptor density across the cell population.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Třepačky
Suché lázně
Fluorometry
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Čtečky identifikačních čipů
Termočipy
Biochemie
Imunoanalýza

