HEK293-CXCR4 Cells

Unit

3 x 10^6 cells/ml

Cell Type

Stable (KO/Expressing) Cells

Tissue Type

Transformed cell lines

Organism

Human

Donor History

Transformed/immortalized; non-tumorigenic (HEK293 background)

Growth Properties

Monolayer

,

adherent

Growth Conditions

Culture medium: RPMI 1640

,

w: 2.0 mM stable Glutamine

,

w: 2.0 g/L NaHCO3 (Cytion article number 820700a). Supplements: Supplement the medium with 10% FBS

,

1 mM sodium pyruvate

,

10 mM HEPES

,

1% NEAA. Add Geneticin (G418-Sulfat) to achieve a final concentration of 1 mg/mL. Dissociation reagent: Trypsin-EDTA. Seeding density: 2 to 4 x 10⁴ cells/cm². Freeze medium: As a cryopreservation medium

,

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

,

or CM-1 (Cytion catalog number 800100)

,

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

,

5% CO2

,

humidified atmosphere.

Immortalization Method
Full Information

https://www.cytion.com/HEK293-CXCR4-Cells/305420

Mgr. Otakar Fišer
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Mgr. Otakar Fišer
Produktový manažer - Life Science
Popis

Disclaimer: The prices displayed for cell lines are exclusively for academic/not-for-profit customers. For commercial entitites the price is approximately €6,250. If you represent a commercial entity or are unsure which category applies, please
contact us.
The HEK293-CXCR4 cell line is a stable recombinant HEK293 cell line engineered to express the CXCR4 receptor at a medium-high level, approximately 7,800 molecules per cell. This cell line was developed using inscreenex’s landing pad technology, which ensures precise and reproducible integration of the CXCR4 gene at a specific, pre-validated genomic locus. CXCR4, also known as CD184, is a chemokine receptor belonging to the G protein-coupled receptor (GPCR) family. It plays a crucial role in immune cell trafficking, hematopoiesis, and serves as a co-receptor for HIV entry into cells. CXCR4 is also implicated in tumor growth, metastasis, and angiogenesis, particularly in hematological malignancies, making it a significant target in cancer research and therapy development.
The expression of CXCR4 in this cell line was confirmed using flow cytometry with a target-specific antibody, ensuring consistent and reliable receptor density across the cell population.

O značce

Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.

Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.