HeLa S3 Cells

The HeLa S3 cell line is a clonal derivative of the original HeLa cell line, which was established from the cervical cancer cells of an adult woman. HeLa S3 cells are notable for their robust growth in suspension cultures and are frequently used in scientific research due to their adaptability to various medium formulations. This variant retains the key characteristics of the HeLa lineage, such as a rapid doubling time and a karyotype that is highly aneuploid, displaying numerous chromosomal abnormalities which are a hallmark of HeLa cells.

HeLa S3 cells are widely utilized in virology, toxicology, and cancer research, particularly because they maintain the ability to be infected by poliovirus and other viruses, making them invaluable in pathogen-host interaction studies. They are also employed in the study of gene expression and regulation mechanisms under physiological and pathological conditions. The genetic and metabolic profiles of HeLa S3 have been extensively characterized, facilitating their use in high-throughput genetic screens and molecular biology applications.