HEP3B Cells

Unit

3 x 10^6 cells/ml

Cell Type

Tumor Cells

Tissue Type

Liver cancer cell lines

Organism

Human

Donor History

Childhood hepatocellular carcinoma

Growth Properties

Adherent

Growth Conditions

Culture medium: EMEM (MEM Eagle)

,

w: 2 mM L-Glutamine

,

w: 2.2 g/L NaHCO3

,

w: EBSS (Cytion article number 820100a). Supplements: Supplement the medium with 10% FBS and 1% NEAA. Dissociation reagent: Accutase. Freeze medium: As a cryopreservation medium

,

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

,

or CM-1 (Cytion catalog number 800100)

,

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

,

5% CO2

,

humidified atmosphere.

Immortalization Method
Full Information

https://www.cytion.com/HEP3B-Cells/305141

SKU: 305141 Kategorie: ,
Mgr. Otakar Fišer
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Mgr. Otakar Fišer
Produktový manažer - Life Science
Popis

The Hep3B cell line, derived from an 8-year-old child with liver cancer, is a pivotal model in the study of human liver cancer cells and their responses to various therapeutic agents. Hep3B cells contain an integrated hepatitis B virus genome and is integral in the investigation of differential drug responses due to its unique genetic and phenotypic characteristics.
The Hep 3B human hepatoma cell line is renowned for its extensive expression of liver-specific proteins such as alpha-fetoprotein (AFP), albumin, and various other markers, making it an invaluable tool in drug metabolism and hepatotoxicity studies. This wide array of expressed proteins allows for a comprehensive assessment of how liver cancer cells interact with and metabolize therapeutic agents.
The Hep 3B cell line and its derivative cell lines enable the tracking of tumor growth and metastasis in vivo, facilitating the study of liver cancer progression and the efficacy of potential treatments.
The Hep3B cell line stands out as a crucial resource for advancing our understanding of liver cancer biology and the development of more effective therapeutic strategies.

O značce

Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.

Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.