Hepa 1-6 Cells

Unit

3 x 10^6 cells/ml

Cell Type

Tumor Cells

Tissue Type

Mouse cell lines

Organism

Mouse

Donor History

Hepatocellular carcinoma

Growth Properties

Adherent

Growth Conditions

Culture medium: DMEM:Ham's F12 (1:1)

,

w: 3.1 g/L Glucose

,

w: 2.5 mM L-Glutamine

,

w: 15 mM HEPES

,

w: 0.5 mM Sodium pyruvate

,

w: 1.2 g/L NaHCO3 (Cytion article number 820400a). Supplements: Supplement the medium with 10% FBS. Dissociation reagent: Accutase. Seeding density: 1 x 10⁴ cells/cm². Freeze medium: As a cryopreservation medium

,

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

,

or CM-1 (Cytion catalog number 800100)

,

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

,

5% CO2

,

humidified atmosphere.

Immortalization Method
Full Information

https://www.cytion.com/Hepa-1-6-Cells/400474

SKU: 400474 Kategorie: ,
Mgr. Otakar Fišer
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Mgr. Otakar Fišer
Produktový manažer - Life Science
Popis

The Hepa 1-6 cell line is a well-characterized model derived from a hepatoma induced in an adult mouse. This cell line is commonly used in biomedical research with a focus on studying liver cancer, liver metabolism, and toxicology. The cells are of epithelial morphology and exhibit an undifferentiated hepatocellular carcinoma phenotype. Hepa 1-6 is particularly valuable for investigating the biochemical pathways involved in liver function and the cellular mechanisms underlying hepatocarcinogenesis.

Hepa 1-6 cells are known for their ability to be cultured easily and maintain stable growth and reproduction under standard laboratory conditions. They express several cytochrome P450 enzymes, making them an excellent tool for pharmacological and toxicological studies. These cells are also used to explore the regulation of gene expression in liver cells and to understand the impact of various substances on liver function. Due to their robust nature and relevance to human liver diseases, Hepa 1-6 continues to be a crucial resource in the field of liver disease research.

O značce

Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.

Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.