Hepa 1-6 Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Mouse cell lines |
| Organism |
Mouse |
| Donor History |
Hepatocellular carcinoma |
| Growth Properties |
Adherent |
| Growth Conditions |
Culture medium: DMEM:Ham's F12 (1:1) ,w: 3.1 g/L Glucose ,w: 2.5 mM L-Glutamine ,w: 15 mM HEPES ,w: 0.5 mM Sodium pyruvate ,w: 1.2 g/L NaHCO3 (Cytion article number 820400a). Supplements: Supplement the medium with 10% FBS. Dissociation reagent: Accutase. Seeding density: 1 x 10⁴ cells/cm². Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
The Hepa 1-6 cell line is a well-characterized model derived from a hepatoma induced in an adult mouse. This cell line is commonly used in biomedical research with a focus on studying liver cancer, liver metabolism, and toxicology. The cells are of epithelial morphology and exhibit an undifferentiated hepatocellular carcinoma phenotype. Hepa 1-6 is particularly valuable for investigating the biochemical pathways involved in liver function and the cellular mechanisms underlying hepatocarcinogenesis.
Hepa 1-6 cells are known for their ability to be cultured easily and maintain stable growth and reproduction under standard laboratory conditions. They express several cytochrome P450 enzymes, making them an excellent tool for pharmacological and toxicological studies. These cells are also used to explore the regulation of gene expression in liver cells and to understand the impact of various substances on liver function. Due to their robust nature and relevance to human liver diseases, Hepa 1-6 continues to be a crucial resource in the field of liver disease research.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Třepačky
Suché lázně
Fluorometry
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Čtečky identifikačních čipů
Termočipy
Biochemie
Imunoanalýza

