HFL1 Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Primary Cells |
| Tissue Type |
Human cells |
| Organism |
Human |
| Donor History | |
| Growth Properties |
Adherent |
| Growth Conditions |
Culture medium: Ham's F12K Medium ,w: 2.0 mM L-Glutamine ,w: 2.0 mM Sodium pyruvate ,w: 2.5 g/L NaHCO3 (Cytion article number 820608a). Supplements: Supplement the medium with 10% FBS. Dissociation reagent: Accutase. Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
The HFL1 cell line, derived from human fetal lung tissue, is commonly used in biological and medical research. These cells exhibit fibroblast-like properties, making them particularly valuable for studies related to cellular morphology, fibrosis, and tissue repair mechanisms. HFL1 cells are instrumental in the exploration of pulmonary diseases, including investigations into the pathogenesis of lung fibrosis and the evaluation of antifibrotic therapies.
In addition to their application in disease models, HFL1 cells are often utilized in pharmacological research and toxicology studies. Their sensitivity to viral infections and responsiveness to pharmacological agents enable researchers to study the effects of various drugs and compounds on lung tissues. The HFL1 cell line supports the propagation of viruses, facilitating studies on viral life cycles and host-virus interactions, which are crucial for the development of antiviral drugs and vaccines.
Overall, the HFL1 cell line is a versatile tool in the fields of respiratory disease research, pharmacology, and toxicology, providing insights into cellular processes and potential therapeutic approaches for lung-related diseases.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Třepačky
Suché lázně
Fluorometry
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Čtečky identifikačních čipů
Termočipy
Biochemie
Imunoanalýza

