HK-CRISPR-mEGFP-RanBP2/Nup358 Cells

The HK-CRISPR-mEGFP-Nup358 cell line is a genetically engineered derivative of HeLa Kyoto cells, known for their robustness and widespread use in scientific research. This cell line has been modified using CRISPR-Cas9 technology to express mEGFP (monomeric Enhanced Green Fluorescent Protein) tagged Nup358, a crucial component of the nuclear pore complex (NPC). Nup358, also known as RanBP2, plays a significant role in nucleocytoplasmic transport, mitotic spindle assembly, and other cellular processes. The mEGFP tag allows for the visualization of Nup358, facilitating real-time observation of its dynamics and interactions within the cell.

HeLa Kyoto cells, a subline of the original HeLa cells, are characterized by their adaptability and stable growth in culture. The CRISPR-Cas9 system in this cell line enables precise genomic editing, ensuring the mEGFP tag is accurately fused to the Nup358 protein without disrupting its function. This makes the HK-CRISPR-mEGFP-Nup358 cell line a valuable tool for studying the structural and functional aspects of the nuclear pore complex. Researchers can use this cell line to gain insights into the mechanisms governing nucleocytoplasmic transport and the role of Nup358 in cellular homeostasis and disease states, such as cancer and viral infections.