HK-CRISPR-NUP205-mEGFP Cells

The HK-CRISPR-NUP205-mEGFP cell line is a genetically engineered human cell line designed to study nucleoporin 205 (NUP205) and its role in the nuclear pore complex. Modified with CRISPR-Cas9 to tag NUP205 with monomeric enhanced green fluorescent protein (mEGFP), it enables visualization and tracking of NUP205 in live cells, aiding research into nuclear transport mechanisms and nuclear pore complex dynamics.
NUP205 is a critical nuclear pore complex component, regulating molecule transport between the nucleus and cytoplasm. Tagging NUP205 with mEGFP allows researchers to observe its localization and behavior in real-time under a fluorescence microscope, making this cell line particularly useful for studying nuclear pore complexes’ structural and functional aspects and their roles in gene expression, RNA processing, and the cell cycle.
The HK-CRISPR-NUP205-mEGFP cell line is a powerful tool for investigating nucleocytoplasmic transport mechanisms and the nuclear pore complex’s role in cellular homeostasis. It is also valuable for exploring how disruptions in nuclear pore function contribute to diseases like cancer and neurodegenerative disorders, offering a robust model for advancing our understanding of nuclear transport and its implications for human health.