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HL-1 Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Mouse cell lines |
| Organism |
Mouse |
| Donor History |
Normal atrial cardiomyocyte (SV40 large T antigen-immortalized via AT-1 lineage; spontaneously contracting; non-tumorigenic in standard use) |
| Growth Properties |
Adherent |
| Growth Conditions |
Culture medium: Claycomb medium ,2 mM glutamine ,10% FBS ,0.1 mM norepinephrine in L-ascorbic acid (We do not sell) Prepare 0.1 mM norepinephrine in L-ascorbic acid: Prepare a 100-fold stock solution = 10 mM norepinephrine. 1. Prepare a 30 mM L-ascorbic acid solution (50 mL) 2. Add 160 mg of norepinephrine to the ascorbic acid solution (50 mL) = 10 mM norepinephrine 3. Sterilely filter the solution through a 0.2-µm filter cartridge. 4. Fill 1-mL aliquots of the 100-fold norepinephrine stock solution into sterile vials and store at −20 °C. Wrap in aluminum foil; must be protected from light. The solution is stable for approximately 1 month at −20 °C. 5. Add 1 mL of this solution to 100 mL of Claycomb medium. The medium is stable for only 1 month. Supplements: Supplement the medium with 10% FBS ,2 mM glutamine ,0.1 mM norepinephrine in L-ascorbic acid. Dissociation reagent: Accutase. Seeding density: 2 to 4 x 10⁴ cells/cm². Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
HL-1 is a cardiac muscle cell line derived from the atrial cardiomyocytes of an adult mouse. This cell line is unique in that it retains the ability to contract and maintain a differentiated cardiac phenotype over long-term culture, making it an invaluable model for cardiovascular research. HL-1 cells are widely used in studies focused on cardiac physiology, electrophysiology, and pharmacology. Their ability to undergo spontaneous and inducible contractions allows researchers to investigate the molecular mechanisms underlying cardiac muscle function, disease, and response to therapeutic agents.
HL-1 cells exhibit characteristics typical of cardiac muscle cells, including the expression of cardiac-specific markers such as troponin, myosin, and connexin 43. They are responsive to various physiological and pharmacological stimuli, enabling detailed studies of cardiac signal transduction pathways, ion channel function, and the effects of drugs on heart cells. This cell line is particularly valuable for modeling cardiac arrhythmias, hypertrophy, and other cardiac pathologies. Additionally, HL-1 cells are used in high-throughput drug screening assays aimed at identifying compounds that modulate cardiac function, which is crucial for the development of new treatments for cardiovascular diseases.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
