HMy2 Cells
| Unit |
5 x 10^6 cells/ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
B lymphoblast cell lines |
| Organism |
Human |
| Donor History |
Plasma cell leukemia |
| Growth Properties |
Suspension |
| Growth Conditions |
Culture medium: RPMI 1640 ,w: 2.0 mM stable Glutamine ,w: 2.0 g/L NaHCO3 (Cytion article number 820700a). Supplements: Supplement the medium with 10% FBS. Seeding density: 1 x 10⁵ cells/mL. Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
The HMy2 cell line is a human B lymphoblastoid cell line derived from an adult individual. This cell line was originally established for the study of human B cell function, lymphoma, and immunological responses. The HMy2 cells are commonly used in research due to their capacity to produce a wide range of immunoglobulins and cytokines, which makes them an excellent model for investigating B cell activation, differentiation, and the molecular mechanisms underlying lymphoid malignancies.
HMy2 cells exhibit typical characteristics of B lymphoblastoid cells, such as a high nuclear-to-cytoplasmic ratio and the presence of surface markers indicative of B cell lineage, including CD19 and CD20. These cells are also known to express HLA-DR antigens, making them suitable for studies related to antigen presentation and immune response modulation. Researchers often utilize HMy2 cells in experiments involving gene expression, transfection, and hybridoma technology, contributing to advancements in therapeutic antibody development and cancer immunotherapy.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Třepačky
Suché lázně
Fluorometry
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Čtečky identifikačních čipů
Termočipy
Biochemie
Imunoanalýza

