HROC222 T1 M2 Cells

HROC222 T1 M2 is a human colorectal adenocarcinoma cell line established within the HROC (Hansestadt Rostock Colorectal Cancer) model collection from a primary tumor resected from an adult patient. The designation “T1” indicates that the specimen was obtained at the first surgical time point, while “M2” denotes the corresponding in vitro model generated from this tumor. The HROC platform integrates comprehensive biobanking, standardized molecular annotation, and parallel establishment of patient-derived xenografts (PDX) and permanent low-passage cell lines, enabling clinically annotated translational research models.

Generation of HROC222 T1 M2 followed standardized procedures involving mechanical dissociation of freshly resected tumor tissue, preparation of single-cell suspensions, and seeding onto collagen-coated culture plates in defined tumor cell culture medium supplemented with glutamine, antibiotics, and antimycotics. Across the HROC cohort, permanent primary colorectal cancer cell lines were successfully established from approximately 13% of attempted specimens. Statistical analysis identified higher tumor grading as significantly associated with successful primary cell line establishment, while advanced nodal status showed a positive trend. In multivariate analysis across the collection, nodal involvement emerged as an independent predictor of model establishment success.

The HROC collection encompasses all major molecular subtypes of colorectal carcinoma, including chromosomal instability (CIN), CpG island methylator phenotype (CIMP), microsatellite stable (MSS), and microsatellite instability-high (MSI-H) tumors, as well as diverse mutational backgrounds affecting key driver genes such as KRAS, BRAF, TP53, APC, and PIK3CA. HROC222 T1 M2 was generated within this rigorously characterized framework, permitting integration with detailed clinicopathological and molecular data and, where available, corresponding PDX material. As a low-passage, patient-derived colorectal carcinoma model, HROC222 T1 M2 is suitable for investigations of tumor biology, genotype-phenotype relationships, and preclinical therapeutic testing within precision oncology research.