Hs-746T Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Gastric cancer cell lines |
| Organism |
Human |
| Donor History |
Gastric adenocarcinoma |
| Growth Properties |
Adherent |
| Growth Conditions |
Culture medium: DMEM ,w: 4.5 g/L Glucose ,w: 4 mM L-Glutamine ,w: 3.7 g/L NaHCO3 ,w: 1.0 mM Sodium pyruvate (Cytion article number 820300a). Supplements: Supplement the medium with 10% FBS. Dissociation reagent: Accutase. Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
The Hs-746T cell line is derived from human gastric carcinoma, representing a valuable in vitro model for studying gastric cancer biology and therapeutic interventions. These cells exhibit epithelial morphology and are known for their adherent growth properties. The Hs-746T cells harbor a MET gene amplification, which is a significant feature as it contributes to the oncogenic signaling pathways involved in gastric cancer. This amplification makes the Hs-746T cell line particularly useful for research focused on targeted therapies against MET and its downstream signaling pathways.
Researchers utilize the Hs-746T cell line to investigate various aspects of tumor biology, including cell proliferation, migration, invasion, and response to chemotherapeutic agents. The genetic and phenotypic characteristics of Hs-746T cells make them an essential tool for studying the molecular mechanisms underlying gastric carcinoma and for the development and testing of new anti-cancer drugs. The availability of this cell line has facilitated numerous studies aimed at understanding the role of MET amplification in cancer progression and treatment resistance, thereby contributing to the advancement of precision medicine in oncology.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Třepačky
Suché lázně
Fluorometry
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Čtečky identifikačních čipů
Termočipy
Biochemie
Imunoanalýza

