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Human Adrenocortical HAC50 Cells
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Adrenal Gland |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,48 ,Adrenal carcinoma |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow IV (TM004) + 10% cosmic calf serum + 1X ITS Plus + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. Selection with 1 µg/ml G418 (G271). |
| Immortalization Method |
Tumor Cell Line |
| Full Information |
https://www.abmgood.com/human-adrenocortical-hac50-cells.html |
Clonal cell line isolated from NCIH295R. The HAC50 cells increase aldosterone production in response to treatment with angiotensin II and high extracellular potassium levels. These cells respond to activation cAMP signaling pathway agonists, forskolin and dibutyryl cAMP, with a time-dependent increase in cortisol and dehydroepiandrosterone production. In summary, the HAC50 cell line is capable of responding to the three main adrenocortical physiologic regulators.
