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Imunoanalýza
Human Plasma Cell Leukemia Cell Line (UHKT-944)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Bone Marrow |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,55 ,Plasma Cell Leukemia |
| Growth Properties |
Suspension ,single cells and clusters |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow II (TM002) + 10% FBS + 1 ng/mL IL-6* (Z100555) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂.*Fresh IL-6 must be added to the culture vessel every 1-2 days during the post-thaw period; once cells have recovered every 2-3 days. Note: Thaw cells in a T25. To subculture cells ,move to new culture vessel and add more media; do not spin cells. Do not clear debris and dead cells from culture vessels as it will negatively impact cell growth. Keep cell density higher. |
| Immortalization Method |
Tumor Cell Line |
| Full Information |
https://www.abmgood.com/human-plasma-cell-leukemia-cell-line-uhkt-944-t8094.html |
This novel, stable plasma cell leukemia cell line (UHKT-944) represents a significant milestone in the study of plasma cell leukemia (PCL). These cells are dependent on exogenous IL-6 for growth. Cell morphology was confirmed through Giemsa staining. UKHT-344 cells express CD138 and CD38 and were negative for the tested B-cell, T-cell and myeloid markers. These cells produce IgA1-kappa without secreting free light chains, as found using nephlometry. IL-6 was found to be essential for both the survival and growth of this cell line. Cells also express weak levels of CD1a, CD9, CD45, CD54, and HLA-DR. This cell line is a suitable model for studies focusing on the cellular and molecular mechanisms of plasma cell leukemia pathogenesis.
