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IFT88+1/-5 CRISPR Knockout ASEC-1 Cell Line – Clone 30
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Embryo |
| Organism |
Lizard (Dactyloidae) |
| Donor History |
Male ,Stage 6 embryo (torso) ,Anolis sagrei |
| Growth Properties |
Adherent ,fibroblast |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. DMEM ,High Glucose Medium (TM500) + 10% HI-FBS + 2mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,29.0°C ,5% CO₂.Note: cells are grown at 29.0°C. Cells are sensitive to trypsin; Gentle Dissociation Solution (TM080) is recommended for subculture procedures. |
| Immortalization Method | |
| Full Information |
CRISPR/Cas9 gene editing was utilized to targeted exon 4 to disrupt the ift88 ORF near the N261 terminus (pSpCas9(BB)-2A-Puro 640 (PX459)) in ASEC-1 cell line. The cells were selected with puromycin (25 µg/ml). Loss of ift88 function in ASEC-1 cells is predicted to prevent ciliogenesis.
