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Imunoanalýza
Immortalized Baby Mouse Kidney Epithelial Cells (iBMK)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Kidney |
| Organism |
Mouse (M. musculus) |
| Donor History |
5-day-old wild-type baby C57B/6 mouse |
| Growth Properties |
Adherent ,cuboidal |
| Growth Conditions | |
| Immortalization Method |
Serial passaging and electroporation transfection with linearized DNA of E1A and p53DD |
| Full Information |
https://www.abmgood.com/immortalized-baby-mouse-kidney-epithelial-cells-ibmk.html |
The Immortalized Baby Mouse Kidney Epithelial Cells (iBMK, Clone W2) is derived by co-expression of adenovirus E1A and dominant-negative p53 (p53DD). In addition to its non-tumorigenicity, this cell line maintains its primary phenotype and expresses epithelial-specific markers such as E-cadherin, cytokeratin 8 and 18. Together with Bax Knockdown iBMK (T3024), Bak Knockdown iBMK (T3025) and Bax/Bak Double Knockdown iBMK (T3026), these cell lines form the ideal system for studying mechanisms regulating apoptosis. In the same way, when paired with Atg5 Knockdown iBMK (T3027) and Atg7 Knockdown iBMK (T3028), they present an attractive model for investigating the relationship between autophagy and metabolism homeostasis. Western blot was used to confirm the presence of E1A and p53DD gene in the immortalized cell line.
