Immortalized Human Cranial Periosteal Cells (Tag58)

The Immortalized Human Cranial Periosteal Cell Line (TAg58) is the first human cranial periosteal cell line isolated from a healthy adult patient biopsy and immortalized by lentiviral transduction with the large SV40 T antigen.The following are key characteristics that have been observed for the Immortalized Human Cranial Periosteal Cell Line (TAg58) (Alexander et al., 2015):Maintains long-term cell proliferation and osteogenic differentiation potentialShows stronger calcium phosphate mineralization than parental primary cellsMaintains similar expression patterns for most surface antigens as parental primary cellsExpresses higher levels of stem cell surface markers, CD146, and MSCA-1 (mesenchymal stem cell antigen-1), as well as higher constitutive mRNA levels of key osteogenesis factors (e.g. Osteocalcin, alkaline phosphatase, runx-2) compared to parental primary cellsThe Immortalized Human Cranial Periosteal Cell Line (TAg58) represents a suitable experimental platform for studying periosteal cell biology, bone formation, and bone regeneration.Depositor Profile:Prof. Dr. Dorothea Alexander-Friedrich, University Hospital TübingenIn Germany alone, more than 10,000 people are newly diagnosed with oral cancer every year resulting in tumor and in the most cases jaw bone resection. In order to reconstruct the resulting bone defects, an alternative to autologous bone transplantation is needed. The research laboratory of the Department of Oral and Maxillofacial Surgery of the University Hospital Tübingen focuses its research on the field of bone tissue engineering (TE). Read how their work with jaw and cranial periosteal cells translate into the generation of 3D TE-constructs and lead the way to biocompatible stem cell-based therapies.Read full profile.Characterization by osteogenic differentiation experiments, Western blot, flow cytometry, detection of cell mineralization by fluorescent osteoImage staining, and gene expression analysis.